Sequence and transfection of BoLA-DRB3 cDNAs.

Autor: Russell, G C, Fraser, D C, Craigmile, S, Oliver, R A, Dutia, B M, Glass, E J
Předmět:
Zdroj: Animal Genetics; Jun2000, Vol. 31 Issue 3, p219-222, 4p
Abstrakt: Summary Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index