| Autor: |
Edman, Peter, Hakansson, Par, Westlund, Per-Olof, Johansson, Lennart B.-A. |
| Předmět: |
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| Zdroj: |
Molecular Physics; 10/10/2000, Vol. 98 Issue 19, p1529-1537, 9p, 2 Diagrams, 1 Chart, 4 Graphs |
| Abstrakt: |
The applicability of an extended Forster theory (EFT) is explored with respect to donordonor energy migration within a pair of identical fluorophores. The EFT accounts for reorienting motions and orientational restrictions of donor groups, which are attached to a macromolecule. Because EFT involves averaging over stochastic functions, it is inappropriate for the conventional methods used for analysing fluorescence depolarization experiments. For this reason approximations of the EFT were derived. To examine the validity of these different approximations, depolarization data were generated and re-analysed. To create the depolarization data, the EFT was used together with a Brownian dynamics simulation. Limitations of the approximate EFT are ascribed to the handling of secondarily excited donors (i.e. donors excited through energy migration). Finally on the basis of the EFT, a simulation-deconvolution method is presented which enables one to analyse the fluorescence anisotropy, without introducing any approximation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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