| Autor: |
Ransom, Evan R., Leibowitz, Jason M., Ma, Cathy, Puttaswamy, Kanchan, Troxel, Andrea, Lee, William, Feldman, Michael, Brose, Marcia S. |
| Zdroj: |
Laryngoscope; Oct2010 Supplement, Vol. 120 Issue S3, pS47-S47, 1p, 1 Color Photograph, 1 Illustration, 1 Graph |
| Abstrakt: |
Objectives: To describe a new technique for assaying effects of antitumor therapeutics over time. Study Design: Tumor tissue samples were taken from a Phase II clinical trial. A method for quantification of immuno-histochemistry (IHC) was developed using a computer algorithm designed to measure staining levels spectrographically. Phospho-ERK was selected as the tumor marker for this demonstration. Results: Average nuclear DAB intensities were 846.972 (pre-treatment) and 229.249 (on-treatment). Staining intensity frequency distribution curves generated showed statistically distinct distributions, both confirming and quantifying the decreased presence of phospho-ERK in the on-treatment sample. Conclusions: A novel technique for quantification of IHC is presented. This process can be used to delineate specific cellular-level responses to targeted anti-tumor therapeutics. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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