| Autor: |
El-Najjar, N. G., Farah, M. J., Hashwa, F. A., Tokajian, S. T. |
| Předmět: |
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| Zdroj: |
Letters in Applied Microbiology; Oct2010, Vol. 51 Issue 4, p456-461, 6p, 2 Black and White Photographs, 1 Chart, 2 Graphs |
| Abstrakt: |
Aim: To study the prevalence and molecular basis of antimicrobial resistance in UPEC. Methods and Results: PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17- aadA5, dfrA1- aadA1, dfrA12-orf5- aadA2 and blaOXA-30- aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern. Conclusions: Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance. Significance and Impact of the Study: These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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