| Autor: |
Dorsam, G., Krieg, Jr., R. J., Chan, W., Ereso, G., Lin, K. -C., Chan, J. C. M. |
| Předmět: |
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| Zdroj: |
Pediatric Nephrology; Jan2000, Vol. 14 Issue 2, p128-131, 4p |
| Abstrakt: |
Male Sprague-Dawley rats (150–200 g) were randomly assigned to sham operation (n=6) or 5/6 nephrectomy (n=12) procedures. Two weeks after the completion of the 5/6 nephrectomy, these animals were again randomly assigned to two groups: non-treatment or treatment with vitamin E supplementation at 200 IU/kg chow. Two weeks later, all animals were sacrificed and the kidneys harvested. The secretory phospholipase A[sub 2] (PLA[sub 2] ) activity was elevated (150%) in the untreated remnant kidney but returned to sham values in the vitamin E-treated kidneys. The cytoprotective heat shock protein (HSP70) and the intracellular antioxidant superoxide dismutase (MnSOD, Cu/ZnSOD) were similar in sham, remnant, and vitamin E-treated remnant kidneys. We conclude that the sudden reduction of renal mass secondary to the 5/6 nephrectomy procedure stimulates PLA[sub 2] activity but not HSP70, MnSOD, or Cu/ZnSOD. This increased activity of PLA[sub 2] in the remnant kidney returned to sham values after vitamin E treatment. The intrinsic cellular antioxidant enzymes, MnSOD, Cu/ZnSOD, as well as the cytoprotective heat shock protein HSP70, showed no significant changes in either vitamin E-treated or untreated kidneys compared with sham. These data are suggestive that the elevation of PLA[sub 2] is a specific and localized response to the sudden reduction of renal mass. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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