| Autor: |
Bayley, Deborah, Sancho, Marie-Rose, Brown, Janet, Brookman, Laurence, Petrak, Karel, Goddard, Peter, Steward, Alan |
| Zdroj: |
Journal of Bioactive & Compatible Polymers; Jan1993, Vol. 8 Issue 1, p51-68, 18p |
| Abstrakt: |
The purpose of this work was to examine the biodistribution of poly( N5-hydroxyethyl-L-glutamine-co-L-glutamic acid) [poly(HEG- co-GA)] after intravenous administration to rats. Poly(γ-methyl-L-glutamate- co-γ-benzyl-L- glutamate) was prepared by ring-opening polymerization of the amino acid N-carboxyanhydride (NCA) derivatives. Sequential removal of the copolymer benzyl groups followed by reaction of the methyl ester groups with 2-amino ethanol gave poly(HEG-co-GA). Three samples of poly(HEG-co-GA) were pre pared with nominal HEG:GA compositions of 50:50, 95:5 and 90:10 mole %, and with weight average molecular weights ( Mw) of 37,000, 50,000 and 110,000 respectively. Following reaction with N-2[4-hydroxyphenyl]ethylamine (tyra mine), the copolymers were radioiodinated with 125 I and administered intra venously to male Wistar rats. 125 I-copolymer tissue distribution was assessed after 10, 15, 30 and 120 min and 8 h. It was found that the copolymer was removed rapidly from the blood compartment and excreted from the body via the kidneys. Only very low amounts of radioactivity were found in the liver and in other organs of the mononuclear phagocyte system. Degradation of the co polymer in vitro, as assessed by size exclusion chromatography (SEC), in plasma and urine over the time scale of the biodistribution experiment ap peared to be minimal. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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