| Autor: |
Maciej Skolimowski, Martin Weiss Nielsen, Jenny Emnéus, Søren Molin, Rafael Taboryski, Claus Sternberg, Martin Dufva, Oliver Geschke |
| Předmět: |
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| Zdroj: |
Lab on a Chip; 8/21/2010, Vol. 10 Issue 16, p2162-2169, 8p |
| Abstrakt: |
A microfluidic chip for generation of gradients of dissolved oxygen was designed, fabricated and tested. The novel way of active oxygen depletion through a gas permeable membrane was applied. Numerical simulations for generation of O2gradients were correlated with measured oxygen concentrations. The developed microsystem was used to study growth patterns of the bacterium Pseudomonas aeruginosain medium with different oxygen concentrations. The results showed that attachment of Pseudomonas aeruginosato the substrate changed with oxygen concentration. This demonstrates that the device can be used for studies requiring controlled oxygen levels and for future studies of microaerobic and anaerobic conditions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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