| Autor: |
Meeyoung Lee, Bunsoon Choi, Hyuk Jae Kwon, Shim, Ju A., Kyung Sook Park, Eun-So Lee, Seonghyang Sohn |
| Předmět: |
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| Zdroj: |
Journal of Inflammation; 2010, Vol. 7, p31-42, 12p |
| Abstrakt: |
Background: It has been suggested that the HLA-G molecule is a genetic risk factor for Behcet's disease (BD). In this study, we evaluated the level of Qa-2, a murine nonclassical class I MHC molecule and possible functional homolog of HLA-G, to determine if it was associated with various symptoms of BD-like mice. In addition, we investigated siRNA (small interfering RNA) treatment to determine if it inhibited Qa-2 expression, thereby changing the symptoms of mice. Methods: RNA interference (RNAi) and vector transfection were employed to manipulate gene expression in vivo in mice. siRNA (small interfering RNA) or Qa-2 expression vector was applied to inhibit or up-regulate Qa-2 expression, respectively. Results: The Qa-2 levels in granulocytes were lower in BD-like mice than in normal controls. The silencing of Qa-2 by intravenous injection of siRNA (500 nmol/mouse, 4 times at 3-day intervals) specifically reduced the Qa-2 levels and worsened the BD-like symptoms. Conclusions: Silencing Qa-2 by injecting siRNA into mice resulted in deterioration of symptoms in BD-like mice. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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