Autor: |
Föll, J. L., Dannecker, L., Zehrer, C., Hettmer, S., Berger, J., Elmlinger, M., Niethammer, D., Ranke, M. B., Dannecker, G. E. |
Předmět: |
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Zdroj: |
Immunology; Jun98, Vol. 94 Issue 2, p173-180, 8p |
Abstrakt: |
The expression of the insulin-like growth factor binding protein-2 (IGFBP-2) was assayed in mononuclear cells originating from different organs of the immune system. All mononuclear cells studied did express IGFBP-2, but the expression level was found to be dependent on the cell type and origin of the cell. T cells showed a higher expression of IGFBP-2 mRNA than did B cells, and CD34+ stem cells expressed IGFBP-2 mRNA at a high level. Expression was highest in bone marrow and thymus. Stimulation of peripheral mononuclear cells resulted in a marked increase of IGFBP-2 mRNA and also intracellular IGFBP-2, as analysed by fluorescence staining. This increase parallels the increase of other known T-cell activation markers. Furthermore, the increase of intracellular IGFBP-2 seems to precede T-cell blast formation and all T cells in active phases of the cell cycle have high levels of IGFBP-2. Our results provide a basis for further investigations on the contribution of the IGF-system to the regulation of T-cell proliferation and differentiation. IGFBP-2, in particular, may have an important influence in the regulation of T-cell activation and proliferation. A 23187, calcium ionophore IGF I/II, insulin-like growth factor-I/II IGFBPs, insulin-like growth factor binding proteins PBMC, peripheral blood mononuclear cell MNC, mononuclear cell MACS, magnetic-activated cell sorting GAPDH, glycerinaldehydphosphate RT-PCR, reverse transcriptase–polymerase chain reaction. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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