| Autor: |
Lai, Chooi-May, Shen, Wei-Yong, Constable, Ian Jeffrey, Rakoczy, Piroska Elizabeth |
| Předmět: |
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| Zdroj: |
Current Eye Research; Nov99, Vol. 19 Issue 5, 12 Black and White Photographs, 2 Charts |
| Abstrakt: |
PURPOSE. This study aimed to investigate the feasibilityof recombinant adenovirus-mediated gene transfer into cells implicated inthe development of choroidal neovascularization (CNV). METHODS. A rat model of CNV which used laser photocoagulation was developed.Gene delivery into the laser spots was investigated following subretinal injectionof recombinant adenoviruses, AdRSVlacZ, AdCMVlacZ or AdCMVgfp. Immunohistochemicalanalysis was performed using a proliferating cell nuclear antigen antibodyand a cytokeratin-specific antibody to identify the cell types transducedby the recombinant adenoviruses. RESULTS. At 7 days post-injection, lacZ expression was detected in 51.6± 13.2% and 71.2 ± 19.3% of laser spots in AdRSVlacZ- and AdCMVlacZ-injectedeyes, respectively. By 28 days post-injection, lacZ expression was only presentin AdCMVlacZ-injected eyes. In vivo fundus fluorescent photographyof AdCMVgfp-injected eyes detected gfp expression in 79.9 ± 12.7% and35.6% ± 19.7% of laser spots at 4 and 7 days post-injection, respectively.Although fundus fluorescent photography did not detect the gfp signal at 10days post-injection, fluorescent microscopy revealed a gfp signal in 81.3± 6.0% of laser spots. Immunohistochemical analysis detected retinalpigment epithelial (RPE) cells as the most predominant proliferating celltype in the laser spots, although several other proliferating cell types werealso identified. X-gal staining showed that the majority of transduced cellswere those present in the laser spots. CONCLUSIONS. It is proposed that following laser photocoagulation, proliferatingRPE cells are susceptible to adenovirus-mediated gene delivery and that theymay be suitable targets for the delivery of antiangiogenic factors by recombinantadenoviruses in order to inhibit developing CNV membranes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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