| Autor: |
Frendo, Pierre, Gallesi, Daniela, Turnbull, Ruth, Van de Sype, Ghislaine, Hérouart, Didier, Puppo, Alain |
| Předmět: |
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| Zdroj: |
Plant Journal; Jan1999, Vol. 17 Issue 2, p215, 5p |
| Abstrakt: |
Summary: Glutathione (GSH) and homoglutathione (hGSH) were quantified inMedicago truncatuladuring plant development. hGSH was detectable only 48 h after seed germination whereas GSH was present in the dry seeds, indicating that only GSH is used for sulphur storage in seeds. The hGSH was detectable only in the underground part of mature plants whereas GSH was present in all the organs. γ-EC synthetase (γ-ECS) and GSH synthetase (GSHS) activities were found in roots and leaves whereas hGSH synthetase (hGSHS) was found only in roots. Full-length cDNA encoding γ-ECS and two partial cDNAs (gshs1andgshs2) showing high identity with GSHS were isolated inM. truncatula. High γ-ECS activity was detected in protein extracts of a γ-ECS-deficientE. colistrain expressing theM. truncatulaγ-ECS. Northern blot analysis showed that the γ-ECS gene was similarly expressed in all the mature plant organs tested, whereasgshs1had a higher expression in leaves and flowers andgshs2was preferentially expressed in roots and nodules. We hypothesise thatgshs1andgshs2encode a GSHS and an hGSHS, respectively.. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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