Polymorphic glutamate dehydrogenase in lilac vitroplants as revealed by combined preparative IEF and native PAGE: Effect of ammonium deprivation, darkness and atmospheric CO2 enrichment upon isomerization.

Autor:	Refouvelet, Eric, Daguin, Florence
Předmět:	DEHYDROGENASES CALLUS (Botany) LILACS POLYACRYLAMIDE gel electrophoresis
Zdroj:	Physiologia Plantarum; Feb99, Vol. 105 Issue 2, p199-206, 8p
Abstrakt:	The activity and polymorphism of glutamate dehydrogenase (GDH) were studied in basal callus of lilac (Syringa vulgaris L.) vitroplants. Native PAGE alone revealed seven bands staggered at regular intervals. Preparative liquid-vein IEF allowed the separation of six to ten GDH fractions with charges ranging between 5.18 and 7.08. Analysis of these GDH fractions in native PAGE indicated that up to seven GDH bands can be detected for each fraction. This suggests the existence of seven isoforms of the enzyme with subunits presenting different isoelectric points. Dark- and ammonium-controlled forms were found to be the more acidic and faster migrating ones in native PAGE. The results support for the first time that atmospheric CO2 enrichment increases GDH activity dramatically and modifies isomerization of the enzyme. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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