| Autor: |
Lee, Jin, Nam, Seong, Park, Hyen, Kim, Young-Min, Kim, Nahyun, Kim, Ghahyun, Seo, Eun-Seong, Kang, Seong-Soo, Kim, Doman |
| Zdroj: |
Food Science & Biotechnology; Jun2010, Vol. 19 Issue 3, p757-762, 6p |
| Abstrakt: |
Appreciably elevated levels of dextranase from Arthrobacter oxydans (AODex) isolated from sugar-cane farm soil was resulted from the culture on the Luria-Bertani (LB) medium containing 1%(w/v) soluble starch, glycerol, or dextran. The responsible gene ( aodex) was cloned, its nucleotide sequence was determined, and expression of the encoded protein was achieved in Escherichia coli. An open reading frame was composed of 1,863 bp putatively encoding a 68.3 kDa protein. Recombinant A. oxydans dextranase (rAODex) was purified about 16 fold by nickel-nitrilotriacetic acid affinity column chromatography; Km value for dextran T2000 was 0.85 mg/mL (w/v). AODex treatment of stale sugar cane juice resulted in a yield of square and light-colored sugar crystals. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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