| Abstrakt: |
Steroid 9α-hydroxylase is a key enzyme system in steroid nucleus degradation in company with Δ1-dehydrogenase. To examine 9α-hydroxylase activity during microbial transformation of steroids, 9(11)-dehydro-17α-methyl-testosterone was adopted as a stable substrate for preventing the rupture of steroid nucleus. Using Nocardia restrictus ATCC 14887 capable of introducing a 9α-hydroxyl group into steroids, 9α, 11α-oxido-17β-hydroxy-17α-methyl-4-androstene-3-one and 9α, 11α-oxido-17β-hydroxy-17α-methyl-1, 4-androstadiene-3-one were obtained. These microbiologically transformed products could be used as reference compounds in the enzyme assay. [ABSTRACT FROM AUTHOR] |
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