| Autor: |
Becker, Hubert, Thellend, Annie, Piffeteau, Annie, Vidal-Cros, Anne |
| Zdroj: |
Glycoconjugate Journal; 2006, Vol. 23 Issue 9, p687-692, 6p |
| Abstrakt: |
Labeled UDP-GlcNAc and chitooligosaccharides should be powerful tools for studies of N-acetylglucosaminyltransferase such as chitin synthases. We describe here a rapid, inexpensive and a common strategie for the chemoenzymatic synthesis of uridine 5′-diphospho- N-[2H]-acetyl-glucosamine and the chemical preparation of N-[2H]-acetyl chitooligosaccharides (from 2 to 5 mers). Deuterated UDP-GlcNAc analogue was tested as chitin synthase substrate and it exhibited an incorporation level in chitin as the natural substrate. Deuterium labeling of carbohydrates present different advantages: it is a stable isotope and allows glycosyltransferase mechanism studies by a mass spectrometry approach. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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