| Autor: |
Koósz, Zs., Gazdag, Z., Miklós, I., Benkő, Z., Belágyi, J., Antal, J., Meleg, B., Pesti, M. |
| Zdroj: |
Folia Microbiologica; Jul2008, Vol. 53 Issue 4, p308-314, 7p |
| Abstrakt: |
A chromate-tolerant mutant chr1-663T bearing a stable one-gene mutation and its parental strain 6chr+ were used to investigate the background of CrVI tolerance in the fission yeast Schizosaccharomyces pombe. The mutant chr1-663T displayed a significantly decreased specific glutathione reductase (GR) activity coded by the pgr1 + gene compared with its parental strain. Transformants of the mutant chr1-663T with a nonintegrative pUR18N vector expressing the pgr1 + gene exhibited the same CrVI sensitivity and specific GR activity as their parental strain, demonstrating the importance of the GR-NADPH system in CrVI tolerance. Transformants, nevertheless, exhibited an increased intracellular peroxide concentration, a decreased CrVI-reducing and HO•-producing ability, which suggested an unbalanced oxidoreduction state of cells and partial complementation of the GR function. No mutation was found in the sequences of the pgr1 + and the pap1 + (transcriptional regulatory gene of GR) genes of the CrVI-tolerant mutant by sequence analysis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink