| Autor: |
Czakó-Vér, K., Koósz, Z., Antal, J., Rácz, T., Sipiczki, M., Pesti, M. |
| Zdroj: |
Folia Microbiologica; Jan2004, Vol. 49 Issue 1, p31-36, 6p |
| Abstrakt: |
Stable chromium(VI)-sensitive and -tolerant mutants were obtained by induced mutagenesis of Schizosaccharomyces pombe lysine and leucine auxotrophic heterothallic strains 6chr + and 9chr +. Eleven of them were selected for further studies. Fast transport of51CrO4 2− was detected in a representative sensitive mutant, chr-51S, while the tolerant mutant chr1-66T and the parental strain 6chr + exhibited significantly lower51CrO4 2− uptake. The segregation of tetrads of three selected CrVI-tolerant mutants, chr1-66T, chr1-14T and chr2-04T, strongly indicated that tolerance was determined by single mutations. Random spore analysis proved that the mutations of chr1-66T and chr1-14T were allelic and the mutation of mutant chr2-04T was not allelic with the mutation of chr1-66T. Recombinants carrying the ura4D18 selective marker were created for transformation experiments. Two of them ( chr1-661T and chr2-046T) can be used to clone and identify the genes responsible for their CrVI tolerance phenotype. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink