| Autor: |
Tashiro, Takuya, Sekine-Kondo, Etsuko, Shigeura, Tomokuni, Nakagawa, Ryusuke, Inoue, Sayo, Omori-Miyake, Miyuki, Chiba, Tomoki, Hongo, Naomi, Fujii, Shin-ichiro, Shimizu, Kanako, Yoshiga, Yohei, Sumida, Takayuki, Mori, Kenji, Watarai, Hiroshi, Taniguchi, Masaru |
| Předmět: |
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| Zdroj: |
International Immunology; Apr2010, Vol. 22 Issue 4, p319-328, 10p, 1 Diagram, 5 Graphs |
| Abstrakt: |
NKT cells are characterized by their production of both Th1 and Th2 cytokines immediately after stimulation with α-galactosylceramide (α-GalCer), which is composed of α-galactopyranose linked to ceramide (itself composed of sphingosine and fatty-acyl chains); the chain length of the ceramide varies and this affects the ability of α-GalCer to stimulate cytokine production. However, the contribution of its galactopyranose sugar moiety remains unclear. We synthesized α-carba-GalCer, which has an α-linked carba-galactosyl moiety; here, the 5a′-oxygen atom of the D-galactopyranose ring of α-GalCer is replaced by a methylene group. The α-carba-GalCer was more stable and showed higher affinity to the NKT receptor. It thus enhanced and prolonged production of IL-12 and IFN-γ compared with α-GalCer, resulting in augmented NKT cell-mediated adjuvant effects in vivo. The α-carba-GalCer, which has an ether linkage, was more resistant to degradation by liver microsomes than was α-GalCer, which has an acetal bond. Modulation of the sugar moiety in glycolipids might therefore provide optimal therapeutic reagents for protective immune responses against tumor or pathogens. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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