| Autor: |
Wintzen, M., de Winter, S., Out-Luiting, J. J., van Duinen, S. G., Vermeer, B. J. |
| Předmět: |
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| Zdroj: |
Experimental Dermatology; Oct2001, Vol. 10 Issue 5, p305-311, 7p |
| Abstrakt: |
The production and its induction by ultraviolet radiation (UVR) of proopiomelanocortin (POMC)-derived peptides by keratinocytes has been reported, albeit not consistently. Recently we demonstrated that only under specific culturing conditions human keratinocytes are capable of producing a β-endorphin (βE)-like peptide with the characteristics of β-lipotropin (βLPH). Here the presence and UV-induction of βE-immunoreactivity (βE-IR) in keratinocytes in human skin in vivo was investigated. βE-IR was detectable by immunohistochemistry in keratinocytes of the follicular matrix and to some extent in cells of sweat ducts, but was absent from epidermal keratinocytes. Absence of βE-IR was confirmed by radioimmunoassay of HPLC-fractionated extracts of normal epidermis. Repeated exposure to solar-simulated UVR had no effect. This investigation is the first to demonstrate the presence of βE-immunoreactive material in the follicular matrix of corporal hairs and in duct cells of sweat glands. The possible meaning of these results is discussed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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