The efficacy of FMD vaccine reduced non-structural proteins with a mAb against 3B protein.

Autor: Dong Li, Zai-Xin Liu, Pu Sun, Yong-Liang Li, Zeng-Jun Lu, Mei-Na Tian, Ying-Li Chen, Bao-Xia Xie, Hui-Fang Bao, Yuan-Fang Fu, Yi-Mei Cao, Ping-Hua Li, Xin-Wen Bai, Jia-Chuan Sun, Jian-Hong Guo, Xiang-Tao Liu, Qing-Ge Xie
Zdroj: Veterinary Research Communications; Jun2010, Vol. 34 Issue 5, p445-457, 13p, 5 Charts
Abstrakt: A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then removing the sepharose by centrifugation. The vaccine was made from the supernatant emulsified with oil-adjuvant ISA206. Ten guinea pigs, 26 pigs and six cattle were vaccinated, and a vaccination control group was included without treatment with 3BmAb-6BFF. After 28 days, 9/10 pigs challenged with FMDV were protected, this result was the same as the control group, indicating that the vaccine potency was not reduced after treatment with 3BmAb-6BFF. The other animals were vaccinated weekly for nine weeks, and serum samples were collected to detect 3ABC-antibody titers. The results showed that 3ABC-antibody production was delayed and the positive antibody rates were lower when vaccination was carried out using vaccines treated with 3BmAb-6BFF compared with untreated vaccines. The findings of this study suggest that it is possible to reduce NSPs using a mAb-sepharose conjugant in FMD vaccines without reducing their efficacy. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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