| Abstrakt: |
Background: Different periodontopathogenic microbiota have been associated with periodontal diseases in several populations. The present investigation determined the subgingival microbiota of untreated chronic periodontitis Brazilians using the checkerboard DNA-DNA hybridization technique. Methods: Twenty-five periodontitis patients (mean age, 41 ± 2; mean probing depth [PD], 3.3 ± 0.2; mean attachment level [AL], 3.6 ± 0.2) with no history of previous periodontal therapy and a control group of 14 healthy subjects (mean age, 34 ± 0.6; mean PD, 1.8 ± 0.2; mean AL, 1.7 ± 0.1) were selected. Measurements of PD, AL, bleeding on probing, plaque accumulation, and suppuration were recorded at 6 sites/tooth Subgingival plaque samples were obtained from 4 sites in each tooth/subject in both groups. The presence and levels of 41 subgingival species were determined in 4,032 plaque samples using whole genomic DNA probes and the checkerboard method. Results: Periodontal pathogens, as well as some unusual species (E. faecalis, E. coli and Bartonella sp.), were detected significantly more often and/or in higher levels in the periodontitis group (P <0.05). Most species were more frequently detected in interproximal sites, B. forsythus, P. gingivalis, E. nodatum, and F. nucleatum ss vincentii showed a significant positive correlation with mean PD and AL (P <0.05). Conclusions: The subgingival microbiota of Brazilians with untreated chronic periodontitis were complex, including high proportions of periodontopathogens commonly found in other populations, as well as some unusual species. [ABSTRACT FROM AUTHOR] |
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