| Autor: |
Zhenxian Gao, Xiaoliang He, Baocun Zhao, Chunjiang Zhou, Yingzhu Liang, Rongchao Ge, Yinzhu Shen, Zhanjing Huang |
| Předmět: |
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| Zdroj: |
Plant & Cell Physiology; May2010, Vol. 51 Issue 5, p767-775, 9p, 1 Diagram, 1 Chart, 7 Graphs |
| Abstrakt: |
High soil salinity is a major abiotic stress in plant agriculture worldwide. Here, we report the characterization of a novel aquaporin gene TaNIP (Triticum asetivum L. nodulin 26-like intrinsic protein), which was involved in salt tolerance pathways in plants. TaNIP was identified and cloned through the gene chip expression analysis of a salt-tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription–PCR (Q-RT–PCR) was used to detect TaNIP expression under salt, drought, cold and ABA treatment. The overexpression of TaNIP in transgenic Arabidopsis produced higher salt tolerance than wild-type plants. Localization analysis showed that TaNIP proteins tagged with green fluorescent protein (GFP) were localized to the cell plasma membrane. Under salt stress treatment, TaNIP-overexpressing Arabidopsis accumulated higher K+, Ca2+ and proline contents and lower Na+ level than the wild-type plants. The overexpression of TaNIP in transgenic Arabidopsis also up-regulated the expression of a number of stress-associated genes. Our results suggest that TaNIP plays an important role in salt tolerance in Arabidopsis and can also enhance plants’ tolerance to other abiotic stresses. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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