Kinetic behavior of phosphotriesterase and its non-covalent complexes with polyelectrolyte in systems with polar and non-polar organic solvents.

Autor: Sergeeva, V.S., Efremenko, E.N., Kazankov, G.M., Gladilin, A.K., Varfolomeev, S.D.
Zdroj: Biotechnology Techniques; Jul1999, Vol. 13 Issue 7, p479-483, 5p
Abstrakt: Soluble phosphotriesterase from E. coli DH5α together with E. coli DH5α cells with the phosphotriesterase activity were co-immobilized into poly(vinyl alcohol) (PVA) cryogel and studied in water/organic systems with polar and non-polar organic solvents. The phosphotriesterase activity was competitively inhibited by polar organic solvents. The inhibition constant correlated with the dielectric constant (ε) of the solvent. The rate of the enzyme-catalyzed reaction in biphasic non-polar solvent/water systems was independent of water/organic ratio and the hydrophobicity of the solvent. Formation of the non-covalent complexes with polyelectrolytes was suggested to enhance the resistance of the phosphotriesterase towards inactivation by organic solvents in their homogeneous mixtures with water. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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