Pulsed column reactor as a novel tool for continuous enzymatic synthesis of peptide in an aqueous/organic biphasic medium.

Autor: Murakami, Yoshihiko, Hayashi, Shigemitsu, Takehara, Akinori, Hirata, Akira
Zdroj: Biotechnology Techniques; Mar1999, Vol. 13 Issue 3, p165-168, 4p
Abstrakt: We propose a novel effective method for a continuous peptide synthesis in an aqueous/organic biphasic medium using a pulsed column reactor. N-Formyl-l-aspartyl-l-phenylalanine methyl ester was enzymatically synthesized continuously. With this extractive method using a pulsed column reactor, we can synthesize peptides with a stable performance even if a peptide (or a peptide-amino acid complex) is precipitated due to its high hydrophobicity. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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