Expression of intercellular adhesion molecule-1 (ICAM-1) during hypoxia-reoxygenation by sinusoidal endothelial cells (SECs) in an obstructive jaundice model.

Autor: Fujii, Masakazu, Noshima, Shinji, Nakayashiki, Chizuru, Hayashi, Daisuke, Inokuchi, Toshihiro, Kobayashi, Tetsuro, Enoki, Tadahiko
Zdroj: Journal of Hepato -- Biliary -- Pancreatic Surgery; Sep2002, Vol. 9 Issue 3, p367-371, 5p
Abstrakt: Background/Purpose: The aim of the present study was to investigate the expression of intercellular adhesion molecule-1 (ICAM-1) by sinusoidal endothelial cells (SECs) during hypoxia-reoxygenation in an obstructive jaundice model. Methods: Male Wistar rats aged 6 to 8 weeks were assigned to an obstructive jaundice group (group OJ; n= 8) and a sham-operated control group (group S; n= 8). Using flow cytometry, we determined the percentage of ICAM-1-positive cells and the mean fluorescence intensity (MFI) of ICAM-1 per cell in each group during hypoxia-reoxygenation. Results: The percentage of ICAM-1-positive cells in group OJ did not change significantly under successive incubation conditions. However, the MFI in group OJ differed significantly between normoxia and 24-h renormoxia. The present study investigated the function of isolated SECs from rats with obstructive jaundice and hypoxia-reoxygenation. The percentage of ICAM-1-positive cells and the MFI of ICAM-1-positive cells showed quite similar changes caused by hypoxia-reoxygenation stress in group S and Group OJ. The ICAM-1 expression increased with hypoxia-reoxygenation in both normal and OJ SECs, and the amount of ICAM-1, on the whole, increased. Obstructive jaundice increased the percentage of ICAM-1-positive cells from the normal state. Conclusions: The amount of ICAM-1 increased with hypoxia-reoxygenation in obstructive jaundice than normal state. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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