| Autor: |
Lüdtke, S., Switek, H., Roth, W., Wild, S., Schmid, J. |
| Zdroj: |
Chromatographia; Jan2002 Supplement, Vol. 55, pS45-S51, 1p |
| Abstrakt: |
We report on a validated assay of a new drug candidate using a 1 mm inner diameter (i.d.) analytical column and semi-automated sample preparation with solid phase extraction in the 96-well plate format. The method was used during a phase-l study in which the results had to be delivered on 102 days within 8 hours. Compared to the original assay using a 2 mm i. d. column, the performance of the new assay is increased. The decrease of the inner diameter from 2 to 1 mm (and the adjustment of the chromatographic conditions) leads to shorter analysis time and enhanced sensitivity without compromising the robutness of the assay. The lifetime of the analytical 1 mm i. d. column was 2500 injections, the one of the guard column about 1300 injections. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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