Utility of Spermidine in PCR Amplification of Stool Samples.

Autor: Kikuchi, Arizumi, Sawamura, Takahiro, Kawase, Naoto, Kitajima, Yasuhiro, Yoshida, Toshiaki, Daimaru, Osami, Nakakita, Takeo, Itoh, Shinichi
Předmět:
Zdroj: Biochemical Genetics; Jun2010, Vol. 48 Issue 5/6, p428-432, 5p, 1 Graph
Abstrakt: The article presents a study examining the detection of the k-ras gene in human stool specimens using conventional polymerase chain reaction (PCR) and SYBR Green I real-time PCR. In this study, human stool samples from healthy subjects were analyzed. Spermidine-containing reaction solutions were used to explore the detection.
Databáze: Complementary Index