| Autor: |
Chaves, A. C., Lucena-Silva, N., Abath, F. G. C., Pereira, V. R. A., Lima-Filho, J. L., Porto, A. L. F., Cabral, J. M. S. |
| Zdroj: |
Bioprocess & Biosystems Engineering; Nov2000, Vol. 23 Issue 5, p435-438, 4p |
| Abstrakt: |
This work describes the partition of a Schistosoma mansoni tegumental antigen produced by a recombinant Escherichia coli strain using an aqueous two-phase system composed of polyethylene glycol (PEG) and potassium phosphate. The effects of the polymer molecular weight, tie line length and pH on antigen partitioning were investigated. The detection of the antigen in both phases were determined by ELISA. The system composed of PEG 3550 (19.7% w/w) and potassium phosphate (17.7% w/w) led to a yield of 59% and an antigen purification factor of 3 in the PEG-rich phase. It was observed that the antigen partition in ATPS was strongly affected by the pH value and tie line length. In addition, it was possible in a single step, to remove the cell debris, that precipitated at the interface of the system. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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