| Autor: |
Kawakami, Makoto, Suzuki, Kimihiro, Matsuki, Yasunori, Hidaka, Toshihiko, Ishizuka, Toshiaki, Konishi, Tatsuya, Takamizawa, Mitsuyo, Kataharada, Koji, Nakamura, Haruo |
| Zdroj: |
Modern Rheumatology; Dec1998, Vol. 8 Issue 4, p401-409, 9p |
| Abstrakt: |
RAMAK-1, a new spontaneously immortalized synovial cell line consisting of type B lining cells, was recently derived from a patient with rheumatoid arthritis (RA). The present study was designed to characterize RAMAK-1 cells in terms of expression of adhesion molecules and cytokine production. RAMAK-1 cells constitutively express intercellular adhesion molecule-1. In contrast, vascular cell adhesion molecule-1 (VCAM-1) expression by the cells was not observed in the absence of stimulation. However, when stimulated by tumor necrosis factor-α, VCAM-1 expression was readily apparent and sustained over 48 h. RAMAK-1 cells spontaneously produced interleukin (IL)-6, IL-8 and granulocyte macrophage colony stimulating factor, but not IL-1α nor IL-1β. However, when stimulated with IL-1β, the cells expressed IL-1α and IL-1β mRNA. This cell line presents functional responses when stimulated with pro-inflammatory cytokines and may be useful in elucidating the initial lesions of the synovial lining layer of RA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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