A comparison of two-dimensional electrophoresis data with phenotypical traits in Arabidopsis leads to the identification of a mutant ( cri1) that accumulates cytokinins.

Autor: Santoni, Véronique, Delarue, Marianne, Caboche, Michel, Bellini, Catherine
Zdroj: Planta: An International Journal of Plant Biology; Apr1997, Vol. 202 Issue 1, p62-69, 8p
Abstrakt: Total proteins extracted from developmental mutants of Arabidopsis thaliana (L.) Heyhn. and from wild-type plants cultivated in the presence of various hormones were analyzed by two-dimensional (2-D) gel electrophoresis. Computer analysis of 2-D gels followed by a statistical treatment of data allowed us to build a phenogram that describes the biochemical distances between the different genotypes. Analysis of the 2-D electrophoresis data allowed us to discriminate mutants in agreement with phenotypical and physiological traits. This biochemical analysis helped us to develop a working hypothesis which led us to show that one developmental mutant ( cri1 ) overaccumulates cytokinins. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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