| Autor: |
Wirsel, S., Horwitz, B., Yamaguchi, K., Yoder, O. C., Turgeon, B. G. |
| Zdroj: |
Molecular & General Genetics MGG; Aug1998, Vol. 259 Issue 3, p272-281, 10p |
| Abstrakt: |
To determine the number of proteins required for mating type ( MAT) locus-regulated control of mating in Cochliobolus heterostrophus, MAT fragments of various sizes were expressed in MAT deletion strains. As little as 1.5 kb of MAT sequence, encoding a single unique protein in each mating type ( MAT-1 and MAT-2), conferred mating ability, although an additional 160 bp of 3′ UTR was needed for production of ascospores. No other mating type-specific genes involved in mating identity or fertility were found. Thus, although homologs of the C. heterostrophus MAT-1 and MAT-2 genes exist in the filamentous ascomycetes Neurospora crassa and Podospora anserina, C. heterostrophus does not appear to have mating type-specific homologs of two additional genes required by both N. crassa and P.␣anserina for successful sexual reproduction. Three genes were identified in the common DNA flanking the MAT locus: a gene encoding a GTPase-activating protein and an ORF of unknown function lie 5′ while a β-glucosidase encoding gene lies found 3′. None of these genes appears to be involving in the mating process. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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