Refined genetic map of the obligate methylotroph Methylobacillus flagellatum.

Autor: Serebrijski, I., Gomelsky, M., Bashkirova, E., Chistoserdov, A., Tsygankov, Y. D.
Zdroj: Molecular & General Genetics MGG; Apr1998, Vol. 258 Issue 1/2, p133-138, 6p
Abstrakt: We present a refined genetic map of the obligate methylotroph Methylobacillus flagellatum. New, Hfr (high-frequency-of-transfer) donors, and pulsed-field gel electrophoresis, were used to determine that M.␣flagellatum contains one ∼3.1-Mb circular chromosome, and no plasmids. A correlation between time-of-entry units and DNA length was established. Using in vivo and in vitro cloning, and sequencing, a number of new genetic markers were identified and mapped; in addition, the nature of some of the previously mapped markers was elucidated. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index