| Abstrakt: |
A rapid method for synthesis and purification of the structured triacylglycerol (TAG), 1,3-dilauroyl-2-oleoylglycerol (LaOLa), has been developed. A fraction containing 70% LaOLa was obtained by enzymatic transesterification between triolein and lauric acid using Lipozyme IM, which has 1,3-regioselectivity on the glycerol moiety of TAG. The fraction was passed through a Bond Elut SI Column to remove mono-and diacylglycerols produced during the transesterification. The TAG fraction thus obtained was applied to a reversed-phase column, eluted with acetonitrile/tetrahydrofuran (8:2, vol/vol) to separate different TAG species. The LaOLa fraction obtained by this method was of greater than 99% purity. The concentrations of total fatty acids and fatty acids bound to the sn-2 position of this LaOLa fraction were determined by gas-liquid chromatography after hydrolysis by pancreatic lipase, to confirm the purity of stereospecific isomers in this fraction. The final purity of LaOLa was found to be greater than 95%, which was in good agreement with the result obtained by high-performance liquid chromatography using a Lichrosorb Si60 argentated column. [ABSTRACT FROM AUTHOR] |
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