Accumulation of Poly (3-Hydroxybutyric Acid) by Some Soil Streptomyces.

Autor: Manna, Anupam, Banerjee, Rebeca, Paul, Amal K.
Zdroj: Current Microbiology; Sep1999, Vol. 39 Issue 3, p153-158, 6p
Abstrakt: In a limited-scale survey, 55 soil streptomycetes were screened for the accumulation of poly (3-hydroxybutyrate) [PHB]. Only 18% of the isolates accumulated PHB ranging between 1.9–7.8% of the dry biomass. The promising isolate DBCC-719, identified as Streptomyces griseorubiginosus, accumulated PHB amounting to 9.5% of the mycelial dry mass in the early stationary phase when grown in chemically defined medium with 2% (wt/vol) glucose as the sole source of carbon. Nitrogen-limiting conditions were inhibitory to growth and PHB accumulation. The isolated polymer was highly soluble in chloroform, gave a sharp peak at 235 nm on digestion with concentrated H2SO4, and had a characteristic infrared spectrum. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index