Changes in Protein Synthesis as a Consequence of Heme Depletion in Escherichia coli.

Autor: Rompf, Alexandra, Schmid, Roland, Jahn, Dieter
Zdroj: Current Microbiology; Oct1998, Vol. 37 Issue 4, p226-230, 5p
Abstrakt: Two-dimensional gel electrophoresis and N-terminal amino acid sequence determination were used to compare the protein synthesis of exponentially growing Escherichia coli with heme-deficient cells. Mutation of the E. coli hemA gene encoding glutamyl-tRNA reductase resulted in the absence of detectable amounts of heme. As a consequence of heme deficiency, the induction of tryptophanase ( trpA), citrate synthase ( gltA), and aldehyde dehydrogenase ( aldA) and the repression of enolase ( eno) and phosphoglycerate kinase ( pgk) were observed. All induced genes are under the control of the catabolite repressor protein Crp. The observed changes in gene expression as a consequence of heme depletion are discussed. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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