| Abstrakt: |
A cell suspension culture assay to determine the phytotoxicity of the fungal toxins phomalide, a host-selective toxin produced by the fungus Phoma lingam (Tode ex Fr.) Desm., perfect stage Leptosphaeria maculans (Desm.) Ces. et de Not., and destruxin B, the major host-selective toxin produced by the fungus Alternaria brassicae (Berk.) Sacc., was carried out with three Brassica spp. It was established that phomalide was significantly less phytotoxic to Cutlass ( Brassica juncea), the cultivar resistant to L. maculans, than to Westar ( B. napus), the cultivar susceptible to L. maculans, at concentrations ≤2×10–5 M. Similar to phomalide, destruxin B, at concentrations ≤5×10–5 M, decreased the viability of cells of the cultivar resistant to A. brassicae (Ochre, Sinapis alba) less than the viability of cells of the susceptible cultivar (Westar, B. napus). Considering the high selectivity of phomalide and its direct correlation with plant disease resistance, phomalide may have great potential application in breeding programs screening/selecting for blackleg resistance in brassicas. [ABSTRACT FROM AUTHOR] |
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