| Autor: |
Siddiqui, Afzal A., Stanley, Christy S., Berk, Steven L. |
| Zdroj: |
Parasitology Research; Mar2000, Vol. 86 Issue 4, p279-283, 5p |
| Abstrakt: |
A full length cDNA encoding the highly immunodominant 41 kDa antigen of Strongyloides stercoralis (P5), recognized by 83% of human patients [Siddiqui et al. (1997) Parasitol Res 83:655–658], is obtained. A clone containing a 1371 bp insert was selected following screening of the S. stercoralis cDNA library with antibodies specific to antigen P5. The nucleotide sequence of this insert identified a cDNA coding for the γ-subunit of isocitrate dehydrogenase (NAD+), GenBank Accession Number AF176568. The conceptually translated amino acid sequence of the open reading frame for the γ-subunit of S. stercoralis isocitrate dehydrogenase (NAD+) encodes a 388 amino acid residue protein with an apparent molecular weight of 43 kDa and a predicted pI of 7.15. The sequence is 71% A/T, reflecting the characteristic A/T codon bias of S. stercoralis. The amino acid sequence of the S. stercoralisγ-subunit of isocitrate dehydrogenase (NAD+) is compared with those of Caenorhabditis elegans, rat and human NAD+-ICDH. The diagnostic potential of the S. stercoralisγ-subunit of isocitrate dehydrogenase (NAD+) is also discussed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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