| Abstrakt: |
A yeast artificial chromosome (YAC) contig from the C57BL/6 ( H2 b) mouse was created from the major histocompatibility complex ( Mhc, H2 in mouse) class Ib subregion, H2-M. It spans approximately 1.2 megabase (Mb) pairs and unites the previous >1.5-Mb YAC contigs (Jones et al. 1995) into a single contig, which includes 21 Mhc class I genes distal to H2-T1. A bacterial artificial chromosome (BAC) contig from the 129 ( H2 bc) mouse, spanning approximately 600 kilobases, was also built from Znf173 ( Afp, a gene for acid finger protein), through Tctex5 ( t-complex testis expressed-5) and Mog (myelin oligodendrocyte glycoprotein), to H2-M2. Twenty-four sequence-tagged site (STS) markers were newly developed, and 35 markers were mapped in the YAC/BAC contigs, which define the marker order as Cen – Znf173– Tctex5 – Mog– D17Tu42– D17Mit232– H2-M3– D17Leh525– H2-M2– Tel. The gene order of Znf173 – Tctex5 – Mog – D17Tu42 is conserved between mouse and human, showing that the middle H2-M region corresponds to the subregion of the human Mhc surrounding HLA-A. [ABSTRACT FROM AUTHOR] |
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