Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.

Autor: McKinney, R., Wentz-Hunter, Kelly, Schmidt, Henning, Potashkin, J.
Zdroj: Current Genetics; Nov1997, Vol. 32 Issue 5, p323-330, 8p
Abstrakt: A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59 and spU2AF23, from fission yeast. We now report a novel U2AF- associated- protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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