| Autor: |
Thier, Ricarda, Delbanco, Evert H., Wiebel, Frederike A., Hallier, Ernst, Bolt, Hermann M. |
| Zdroj: |
Archives of Toxicology; Dec1998, Vol. 72 Issue 12, p811-815, 5p |
| Abstrakt: |
A new system has been developed to determine enzyme activities of glutathione transferase θ (GSTT1-1) based on radiometric product detection resulting from the enzymic reaction of methyl chloride with 35S-labelled glutathione. In principle, the method is universally applicable for determination of glutathione transferase activities towards a multiplicity of substrates. The method distinguishes between erythocyte GSTT1-1 activities of human `non-conjugators', `low conjugators' and `high conjugators'. Application to cytosol preparations of livers and kidneys of male and female Fischer 344 and B6C3F1 mice reveals differential GSTT1-1 activities in hepatic and renal tissues. These ought to be considered in species-specific modellings of organ toxicities of chlorinated hydrocarbons. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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