Detection of BHV-1 in a Naturally Infected Bovine Fetus by a Nested PCR assay.

Autor: Rocha, M.A., Barbosa, E.F., Guedes, R.M.C., Lage, A.P., Leite, R.C., Gouveia, A.M.G.
Zdroj: Veterinary Research Communications; Mar1999, Vol. 23 Issue 2, p133-141, 9p
Abstrakt: Bovine herpesvirus 1 (BHV-1) is frequently associated with abortion in naturally and experimentally infected cattle. Most of the virus isolation and immunofluorescent antibody protocols described in the literature for detecting BHV-1 in bovine foetuses are rather laborious, costly and time-consuming. The detection is described of BHV-1 in the tissues of a naturally aborted bovine foetus by a nested PCR assay with no further hybridization procedures. Optimal results were achieved by filtering the foetal tissues on a chromatography column before DNA extraction, by using two pairs of primers in a nested PCR and by evaluating the amplification products on silver-stained polyacrylamide gels. This nested PCR was faster and easier to perform than the virus isolation test. To our knowledge, this is the first time that BHV-1 has been detected in the tissues of a naturally infected bovine foetus by means of a nested PCR. The test seems to be a practical alternative for rapid detection of BHV-1 in bovine foetus. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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