Monoclonal Anti-GFAP Antibodies: Extraction, Characteristics, and Immunoenzyme Assay.

Autor: Chekhonin, V., Gurina, O., Dmitrieva, T., Shepeleva, I., Ryabukhin, I., Babich, G.
Zdroj: Bulletin of Experimental Biology & Medicine; Aug2001, Vol. 132 Issue 2, p772-775, 4p
Abstrakt: Methods of GFAP purification and obtaining of hybridoma cells producing monoclonal anti-GFAP antibodies and properties of GFAP preparation were described. The immunobloting data on specificity of obtained monoclonal antibodies are presented. A new method of GFAP immunoenzyme assay based on GFAP preparation and anti-GFAP antibodies was elaborated. Standardization of the immunoenzyme system was shown in tests for specificity, accuracy, and reproducibility. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index