Cell Culture Test System for Express Analysis of Cytotoxic and Growth-Stimulating Effects of Bioactive Compounds.

Autor: Chernikov, V., Terekhov, S., Krokhina, T., Shishkin, S., Smirnova, T., Adnoral, N., Rebrov, L., Denisov-Nikol'skii, Yu., Bykov, B.
Zdroj: Bulletin of Experimental Biology & Medicine; Jun2001, Vol. 131 Issue 6, p573-575, 3p
Abstrakt: Cultures of human and mammalian cells presenting 4 types of differentiation (normal human fibroblasts and myoblasts, human and Syrian hamster hepatoma cells, and mouse/mouse hybridoma cells) were used in a panel biotest system. This system allowed to evaluate the cytotoxic and stimulatory effect of bioactive compounds by determining the dose-effect relationships and some quantitative parameters including LD50. Examination of some biolactive compounds of different nature (sangviritrin, escin, deltostim, cycloheximide, dexamethasone) confirmed high efficacy of this biotest system. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: