| Abstrakt: |
Objectives and Design: The effect of mycobacterial lipids on the onset of the early acute inflammatory response in BALB/c mice pleurisy was investigated.¶ Materials and Methods: Intact Mycobacterium leprae and Mycobacterium bovis BCG (BCG), their lipids, and delipidated mycobacteria were used to evaluate total leukocytes and cell types migrated to the pleural cavity (8 animals/ experimental group).¶ Results: BCG Moreau (× 10-6/cavity), delipidated BCG and its lipids gradually recruited cells leading to arrival, respectively, of neutrophils (7.8 ± 1.9, 4.7 ± 0.9, 1.8 ± 0.25) followed by mononuclear cells (4.8 ± 0.8, 3.7 ± 0.7, 2.45 ± 0.22) and eosinophils (0.39 ± 0.08, 0.32 ± 0.11, 0.41 ± 0.65). BCG delipidation decreased the number of migrated total leukocytes (ANOVA, and Newman-Keuls-Student-test), whereas M. leprae delipidation accumulated neutrophils (0.85 ± 0.01) and eosinophils (1.65 ± 0.18).¶ Conclusions: Intact M. leprae and its lipids did not incite any cell recruitment. Apolar external cell wall lipids from M. leprae and BCG induce different cellular responses. They seem to have a crucial importance at the first contact of mycobacteria with the host cell, modulating the influx of neutrophils/macrophages in the early (4/24 h) onset of the inflammatory reaction. [ABSTRACT FROM AUTHOR] |
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