| Autor: |
Lubavina, I., Salomatina, I., Zinchenko, A., Zherdev, A., Dzantiev, B. |
| Zdroj: |
Russian Journal of Bioorganic Chemistry; Mar2000, Vol. 26 Issue 3, p207-212, 6p |
| Abstrakt: |
Detecting labels based on water dispersions of colloidal textile dyes were developed that are useful in various analytical and diagnostic test systems for a simple visual assessment of the assay. Colored water-insoluble particles of dyes were used for the sorptional immobilization of streptavidin on their surface. The resulting streptavidin-dye (STR-DYE) complexes possessed a high visualizing capacity and were used for the combined detection of pesticides (simazine and 2,4-dichlorophenoxyacetic acid) by noninstrumental immunoassay (DYE-comb-assay, competitive dot-immunoassay in the comb format). The detection limits and the duration of our DYE-comb-assay (4 ng/ml, 20–25 min), HRP-comb-assay (competitive dot-immunoassay in the comb format using the enzymic conjugate of STR with horseradish peroxidase) (16 ng/ml), and the traditional competitive ELISA (12–16 ng/ml, 1.5 h) were compared. This DYE-comb-assay is simple enough and can be used under field conditions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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