| Autor: |
Bakunina, I., Kuhlmann, R., Likhosherstov, L., Martynova, M., Nedashkovskaya, O., Mikhailov, V., Elyakova, L. |
| Zdroj: |
Biochemistry (00062979); Jun2002, Vol. 67 Issue 6, p689-695, 7p |
| Abstrakt: |
An α-N-acetylgalactosaminidase IV able to remove blood type specificity of human A(II)-erythrocytes and not effecting B(III)-erythrocytes was isolated from the marine bacterium Arenibacter latericius KMM 426T. The α-N-acetylgalactosaminidase IV preparation exhibits high activity during inhibition of hemagglutination with blood group substance A containing determinants analogous to A-erythrocytes. The enzyme has a pH optimum from 7.0 to 8.0 and completely retains its activity during 30-min heating at 50°C and for a week at 20°C. The enzyme can be stored under the sterile conditions for any length of time at 4°C, but it does not withstand freezing. The α-N-acetylgalactosaminidase is resistant to NaCl; for p-nitrophenyl-α-N-acetyl-D-galactosaminide, the Km is 0.38 mM. The molecular mass of the enzyme determined by gel filtration is 84 kD. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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