| Autor: |
Navolotskaya, E., Malkova, N., Zargarova, T., Lepikhova, T., Krasnova, S., Lipkin, V. |
| Zdroj: |
Biochemistry (00062979); Mar2002, Vol. 67 Issue 3, p357-363, 7p |
| Abstrakt: |
β-Endorphin and the synthetic β-endorphin-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (referred to as immunorphin), corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain, were shown to stimulate concanavalin A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met5]Enkephalin and the antagonist of opioid receptors naloxone examined in parallel were inactive. The stimulating effect of β-endorphin and immunorphin on T lymphocyte proliferation is not inhibited by naloxone. Studies on receptor binding of 125I-labeled immunorphin to T lymphocytes revealed that it binds with high affinity to naloxone-insensitive receptors ( Kd = 7.0 ± 0.3 nM)). Unlabeled immunorphin completely inhibits 125I-labeled β-endorphin specific binding to naloxone insensitive receptors on T lymphocytes ( Ki = 0.6 ± 0.1 nM)). Thus, β-endorphin and immunorphin interact with common naloxone insensitive receptors on T lymphocytes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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