Autor: |
Sinitskaya, N., Shataeva, L., Potekhina, T., Kas'yanov, A. |
Zdroj: |
Applied Biochemistry & Microbiology; Jan2001, Vol. 37 Issue 1, p23-28, 6p |
Abstrakt: |
Complexes of nucleic acids and acid nuclear proteins that are active toward human T-lymphocytes were isolated from cells of baker's yeast Saccharomyces cerevisiae. The conditions of isolation of nucleoprotein complexes by acid extraction followed by microfiltration for concentration of macromolecular components were optimized. Gel filtration and electrophoresis were used to study the composition and molecular weights of components of the preparations obtained. It was shown that the nucleoprotein complex had a molecular weight of 1430 kDa. However, only one zone was determined by electrophoresis of the protein component with a molecular weight of 30 kDa. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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