Production of antibodies to roridin and their applications.

Autor: Burkin, A., Kononenko, G., Zoryan, V., Soboleva, N., Zotova, E.
Zdroj: Applied Biochemistry & Microbiology; Jul2000, Vol. 36 Issue 4, p368-371, 4p
Abstrakt: Polyclonal rabbit antibodies against a conjugate synthesized through condensing BSA and disubstituted roridin A hemisuccinate allowed roridin A to be determined in solutions at a sensitivity of 0.2 ng/ml. The cross-reactivity of structural analogues—roridin A, verrucarin A, and verrucarol—amounted to 100, 2.5, and 0.03%, respectively. The data showed that these antibodies determine roridin A in an indirect heterogeneous enzyme immunoassay in cereal straw samples at a sensitivity of 20 μg/kg. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index