Complete sequence of p53 gene in 20 patients with lung cancer: comparison with chemosensitivity and immunohistochemistry.

Autor:	Brattström, Daniel, Bergqvist, Michael, Lamberg, Kristina, Kraaz, Wolfgang, Scheibenflug, Lena, Gustafsson, Gunnar, Inganas, Mats, Wagenius, Gunnar, Brodin, Ola, Brattström, D, Bergqvist, M, Lamberg, K, Kraaz, W, Scheibenflug, L, Gustafsson, G, Inganäs, M, Wagenius, G, Brodin, O
Předmět:	ANTINEOPLASTIC agents PROTEIN metabolism COMPARATIVE studies DNA DRUG resistance DRUG resistance in cancer cells IMMUNOHISTOCHEMISTRY LUNG cancer LUNG tumors RESEARCH methodology MEDICAL cooperation GENETIC mutation ONCOGENES RESEARCH EVALUATION research SEQUENCE analysis
Zdroj:	Medical Oncology; Dec1998, Vol. 15 Issue 4, p255-261, 7p
Abstrakt:	In this study the entire p53 complementary DNA has been sequenced in 20 non-small cell lung carcinomas (NSCLC) and the results correlated with chemosensitivity, immunohistochemistry and clinical data. Ten patients had mutations in p53, 8 missense mutations and 2 nonsense mutations. The method discovered two mutations never described previously and two other mutations that have never been described before in connection with NSCLC tumours. Chemosensitivity data, according to a short-term assay (FMCA), indicated that tumours with p53 mutation were more resistant to cisplatin and cyclophosphamide. Immunohistochemical studied demonstrated a 70% concordance between over-expression of p53 protein and mutation in p53. No conclusions or trends could be drawn from the immunohistochemical studies of Bcl-2 and Bax. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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